| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermus thermophilus | TACGCCTACTCCGCAAACC | AGCTTGCGCTTGAGGTAGG | 59.78 | 60.08 | 176 | 96 |
100.00%
|
100.00%
|
| Primer 2 | Thermus thermophilus | ATCCTGCGCTTCAACGAGG | ACGCAGACGATCTTGGTGG | 60.45 | 60.08 | 152 | 94 |
100.00%
|
100.00%
|
| Primer 3 | Thermus thermophilus | GAGTTCCCCAGCTTGAGAGG | TGAACCCATCGGTGACCAC | 59.75 | 59.63 | 298 | 93 |
100.00%
|
100.00%
|
| Primer 4 | Thermus thermophilus | ATCATGGTGCAAGGGGTGG | AAGCCCTCGGTGAAGCTTC | 60.00 | 60.00 | 227 | 87 |
100.00%
|
100.00%
|
| Primer 5 | Thermus thermophilus | TTACGAGCAACGCCTCCTG | GCGCTTGAAGTTGTCCGTG | 60.08 | 60.08 | 223 | 87 |
100.00%
|
100.00%
|
| Primer 6 | Thermus thermophilus | TTTGAGCCCTTCGTCCTGG | GGCGATGTTCTTGGCGTTC | 59.63 | 59.87 | 222 | 87 |
100.00%
|
100.00%
|
| Primer 7 | Thermus thermophilus | ATGGTTGGGGTCTTCCTGC | AAAGCCCAAGAGGACCTGG | 59.62 | 59.23 | 168 | 82 |
100.00%
|
100.00%
|
| Primer 8 | Thermus thermophilus | TCTTCCTCCTCACCGTGGA | GCTCTCCACGGCAAGAACT | 59.54 | 60.00 | 193 | 81 |
100.00%
|
100.00%
|
| Primer 9 | Thermus thermophilus | ACCACCACCATCCTCTTGC | TGGTGTAGCGCAGGTTCTC | 59.62 | 59.71 | 175 | 79 |
100.00%
|
100.00%
|
| Primer 10 | Thermus thermophilus | TCCTCTTGAACACCGTGGC | AAGGGGAGGGTGAGGAAGAG | 59.93 | 60.25 | 160 | 76 |
100.00%
|
100.00%
|